Filip Duša

Analytical Chemistry Group, Van ’t Hoff Institute for Molecular Sciences, University of Amsterdam

Deputy Head

Filip Duša graduated in biochemistry from Masaryk University in Brno in 2010. During the following PhD studies at the Institute of Analytical Chemistry of the CAS, he focused on the multidimensional separation methods by coupling isoelectric focusing and liquid chromatography. Further, he focused on the analysis of nanoparticles – liposomes by analytical and biosensing methods during his postdoctoral stay with Prof. Susanne Wiedmer at the Department of Chemistry, University of Helsinki. In 2015 he was awarded a postdoctoral internship by the CAS and returned to his home institute. He also received an award under the Research and Mobility Support Programme for Early Career Researchers, which enabled him to further develop his collaboration with the team in Helsinki and continue his work on liposome analysis. Since then, his main research topics have been capillary electrophoresis, isoelectric focusing, quartz crystal microbalance, nanoplasmonic sensing, and biomimicking nanoparticle analysis. He supervises bachelor, master, and PhD students and periodically teaches isoelectric focusing at Masaryk University.

Resolving Surface-Charge Density Distributions in Polymeric Nanoparticles Using CZE, HDC, and Chemometric Deconvolution

Understanding nanoparticle surface charge is crucial for optimizing their performance in industrial and biomedical applications. Using capillary zone electrophoresis (CZE) and hydrodynamic chromatography (HDC) with chemometric deconvolution, we resolved detailed surface charge density (SCD) distributions in polymeric nanoparticles. Our approach revealed size-dependent variations, multimodal charge populations, and the impact of surfactants on bare NP characterization.

Section title

IChemistry research that matters

The mission of the Van 't Hoff Institute for Molecular Sciences (HIMS) is to push the boundaries of chemistry by performing internationally recognized scientific research that is curiosity driven as well as application driven. HIMS strives at utilization and expansion of its knowledge and expertise by engaging in collaborative research efforts that address challenges in society and industry.

HIMS is one of eight research institutes of the Faculty of Science (FNWI) of the University of Amsterdam. HIMS houses around 200 researchers (145 fte) with over 30 nationalities. Together they publish around 200 peer reviewed scientific articles, 20 PhD theses and on average 4 patent applications per year.