Filip Duša

Department of fluid phase separations, Institute of Analytical Chemistry Czech Academy of Sciences

Deputy Head

Filip Duša graduated in biochemistry from Masaryk University in Brno in 2010. During the following PhD studies at the Institute of Analytical Chemistry of the CAS, he focused on the multidimensional separation methods by coupling isoelectric focusing and liquid chromatography. Further, he focused on the analysis of nanoparticles – liposomes by analytical and biosensing methods during his postdoctoral stay with Prof. Susanne Wiedmer at the Department of Chemistry, University of Helsinki. In 2015 he was awarded a postdoctoral internship by the CAS and returned to his home institute. He also received an award under the Research and Mobility Support Programme for Early Career Researchers, which enabled him to further develop his collaboration with the team in Helsinki and continue his work on liposome analysis. Since then, his main research topics have been capillary electrophoresis, isoelectric focusing, quartz crystal microbalance, nanoplasmonic sensing, and biomimicking nanoparticle analysis. He supervises bachelor, master, and PhD students and periodically teaches isoelectric focusing at Masaryk University.

Development of isoelectric point markers for highly sensitive isoelectric focusing separations

Isoelectric focusing, particularly in the capillary isoelectric format, is a robust technique for theseparation of protein isoforms and bioparticles. The precise calibration of the isoelectric points of theseparated analytes necessitates a comprehensive and accessible array of internal standards (markers).Over the past decade, we have presented an extensive collection of developed markers, comprising overa hundred selectively detectable markers. In addition, a set of novel fluorescent markers has beendeveloped to facilitate highly sensitive analysis of fluorescent and fluorescently labeled analytes

Section title

Institute of Analytical Chemistry of the Czech Academy of Sciences

The department of Fluid Phase Separations (FPS) is one of the five departments of the Institute of Analytical Chemistry of the Czech Academy of Sciences based in Brno, Czech Republic. The efforts of the institute aim toward utilization of the analytical chemistry in diverse branches of science and technology including medicine, environmental protection, foodstuff production, and high-purity materials. We engage in developments of theoretical background, applications, and instrumentation of separation and spectroscopic methods of analytical chemistry.